Tissue Culture Microscope
Jul/100
Tissue Culture Microscope
One of the most important uses of deionised water is in the laboratory. May it be a chemical, microbiological, genetics and molecular biology, medical or Tissue Culture laboratory, water is an indispensable item inside the lab.
These are areas of science which call for the one of purest form of water available known as deionised water.
The process involved in deionising water is long and painstaking, ensuring that the end product is uncontaminated and free from impurities. It undergoes a five step process of filtration: organic trap, twin bed deionizer, mixed bed resin, Ultra Violet treatment, and filtration through a 0.02 micrometer pore size.
Organic impurities are filtered out first then the ions (cations and anions) are removed. At this point, water is deionised. However, there are still other impurities found in water and so water is subjected to an ultraviolet treatment to kill microorganisms. Another filtration through a mixed bed resin is used to remove any remaining impurities. Finally, water is filtered using a filter of a 0.02 pore size.
The end product is ideal for use in the laboratory wherein every single item must be in their purest form to come up with accurate results.
In the chemistry laboratory, it is used in preparing glass wares, utensils, and other tools. Normal or tap water usually has impurities present, such as fluorine and chlorine, which can influence the results of your chemistry experiments.
Deionised water is used to rinse glass wares, laboratory tools, as well as small and large apparatus used in the microbiological testing. It is also used as a solvent in media preparation and other microbiological procedures requiring water. Everything must be sterile inside the microbiology lab and every process must be done asceptically.
In a molecular biology lab, deionised water needs to undergo further filtration to be DNase or RNase-free to be deemed usable in this setting. Molecular biology and genetic experiments are particularly sensitive to impurities because of the need for an accurate and exact set of results. The standard error must be very slim indeed.
The same goes for a tissue culture laboratory, everything must be sterile and the processes must be asceptic. Deionised water is also used in media preparation as well as in the set-up of the needed items and equipment.
A medical laboratory processes clinical specimen of patients to obtain more information on his/her health. The physician can then make a better evaluation of the patient's health based on the results of a urine sample or a blood sample, for instance. Therefore, careful handling and preparation of the samples and the whole test are performed to ensure that the results are accurate. The proper diagnosis of a patient's health condition lay heavily on the results these tests will show.
Deionised water, however, must meet the standards for each type of laboratory since each also has a different need. Nonetheless, deionised water is already of a higher grade than tap water and it can be used for various laboratory preparations and procedures.
Aside from its crucial uses in the laboratory setting, deionised water is also used extensively in the food processing, pharmaceutical, cosmetics, electronics, and automotive industries. It has even found its use into your local carwash. Water is indeed a central ingredient in everything we do.
Jo is a writer for 'The-Water-Company.com', a reputable UK stationed high quality water manufacturer for more than thirty years, supplying products like deionized water and demineralized water to a wide variety of customers in UK, Europe and the rest of the world. If your business has a laboratory water supply requirement for use in calibrating, for testing and analysis of water soluble elements or for cleaning laboratory equipment and glassware then take a look at The-Water-Company.com.
science reasearch culture, tissue samples?
Is there a place that sells living samples in petri dishes of human diseases such as cancer cells. I would like to view them under a microscope and try to find ways to destroy them. I am not a scientist or researcher. This is just something that interests me.
Thank You
ATCC is the main place researchers=scientists go when they purchase cell lines for most diseases. The people who generate new lines, such as cancer cell lines etc., will most likely donate them to this repository for all researchers to use. It costs money, from $250-500, for each cell line. If you are truly interested volunteer in a Research Lab, the reason for this is related to money. Most labs are always glad to get interested people to join them, even with no background in biology or science. This is the level where most scientists start out there career in college anyways.
Biology is extraordinarily expensive! Let's start with getting your lab up and running. The incubators, refridgerators, tissue culture hoods, and everything else you would need (all sterile) for only cell-culture run around $15,000 if you are good at negotiating. This does not include utilities, or set-up (air-filtration system, biohazard disposal etc.) for any of these large products ( 8ft tall, 8ft wide, 3ft deep, and up to 1,000lbs) and is just for Cell Culture. The running cost each month would be well over a thousand dollars. Next comes a good scope, easily $20,000 for a good disecting scope. Want fluorescent filters and a laser to do any kind of histology, up it to $50,000. Digital Camera? Add another few thousand. You need a computer to visualize your data, get a G5=$2,000+. Any real cell biology or molecular biology, another $5,000-$10,000 for reagents and some micropipets. Tack on about $2,000 for running monthly expenses as most things like pipet tips you cannot reuse and must be sterile. Centrifuges? You will need them for anything with molecular biology, another couple thousand dollars. You will need flammable storage and hazardous waste disposal method, plus a Fume Hood for some of this stuff. Add $5,000. Remember none of this includes powering all this crap 24/7.
Bioptechs Delta T Open Dish System
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Microplate Washer
Jun/100
Microplate Washer
Microplate Readers And Washers For Laboratories
Microplate readers and washers are used for a variety of laboratory purposes. Microplate readers are designed to scan, analyze and obtain numerical results from chemical reactions conducted in microplates, whereas microplate washers are designed to wash immunoassays in microwell strips and plates with professional accuracy. IAD filters are used in both microplate readers and washers.
Microplate Readers with Sophisticated Features
Stat Fax 3200 Microplate Reader, Stat Fax 2100 Microplate Reader and Stat Fax 4200 Multichannel Microplate Reader are the various models available. These perform data reduction calculation such as regression, curve fitting and cut off absorbance calculation. They offer automatic reading. Bichromatic optics and four filters are unique features. Larger display with graphics capability is another attractive feature. These can store around 100 user tests in a large non-volatile memory. Other features are:
• PC Recorder software for PC interface
• Optional onboard printer
• Parallel and serial output
• Interactive touch screen LCD with USB mouse option
Highly Efficient Microplate Washers
Microplate washers are also available in a variety of models -- Stat Fax 2600 Microplate Washer, Stat Fax 2600+ Microplate Washer, Microplate Stat Wash 3100 Manual Washer and Dyntex Ultrawash Plus Microplate Washer. Microplate washers wash flat, round and V- bottom plates and strips automatically. When the device is in operation, no user adjustments are required as it offers automatic calibration, alignment and last row detection. Large non-volatile memory stores a minimum of 50 user-defined wash protocols. These washer models are furnished with constant monitoring of vacuum and pressure for precise operation. Quiet operation is ensured through pump cycle. Other features are programmable automatic rinse cycle, bottles, aerosol shield and 8 way main fold.
Excellent Options for Your Laboratory
Microplate readers and washers are great additions to your laboratory. However, before making a purchase, take care to identify products with better performance, reliability and cost effectiveness. Awareness Technology is a leading manufacturer in this field offering quality products that are reasonably priced. NTRL and CE certified microplate readers and washers for laboratories are the best choice.
About the Author
Block Scientific is the affordable source of fine Medical Laboratory Equipments like Centrifuges, Chemistry Analyzers, Microplate Readers and Washers, Blood Gas Analyzers and more.
Semi-Automated ELISA Liquid Handling using EL406 Combination Microplate Washer Dispenser from BioTek
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Microscope Heavy
Jun/100
Microscope Heavy
If you have ever noticed a bird flying, you will see how it uses its inherent ability to rightly use the aerodynamic forces to fly high in the sky as well as glide through the clouds tirelessly and effortlessly. I use these principles in a myriad of exciting homeschool science experiments, some of which I will share below.
These 4 aerodynamic forces are thrust (the force that makes a bird move forward), drag (the force of resistance which the bird has to minimize), lift (the force that makes the bird rise high) and gravity (the force that pulls the bird down). A slight imbalance in the above- and the flight would not be as graceful.
It is important to note that the force of lift and thrust is generated when the birds flap their wings. They also create a lift while gliding against a current of wind by tilting the front edges of the wings to create a difference in air pressure. Drag is minimized by the aerodynamic shape of the bird's body. Also, birds have hollow bones, making them lighter and reducing the effects of gravity. I will share some of my favorite homeschool science experiments below to demonstrate how objects rise in the air and why flying objects must be light in weight.
Airplanes and rockets are designed with these principles in mind. One or more of the above mentioned forces are dominant depending on the function of the flying object. Even if you and I had wings, we wouldn't be able to fly since our bodies are not aerodynamically shaped and we are heavy.
Now that you have learned something about aerodynamics, let's have fun with a hot air balloon experiment. If you've ever been in a hot air balloon, you will notice that the pilot adjusts the burner in order to make the balloon go upwards and downwards. He also uses the winds, caused by a difference in air pressure, to move from place to place. Let's make our own hot air balloon using a garbage bag.
Hot Air Balloon Experiment: Open up a garbage bag and make the mouth very narrow using duct tape. Make sure to leave a small hole to fill-in hot air. Now run a hair dryer for a few seconds till it blows hot air and then fill the bag with hot air. Now switch off the hair dryer and let go of the bag. The bag begins to rise towards the ceiling, where it stays for some time.
Hot air has less density. This means that there are fewer air particles inside the hot air balloon as compared to the outside atmosphere. Therefore there is less air pressure inside the hot air balloon as compared to the outside air. This makes the balloon rise.
Tissue Parachutes: Take four pieces of thin strings or sewing thread of equal lengths. Tie the threads to the four corners of a tissue paper. Tie the free ends of the four strings to a piece of stick. Prepare many such parachutes with different weights attached. Now drop them one by one from a balcony. Notice how fast each one drops to the ground.
Do you now realize why it is important for birds and airplanes to be light in weight?
The free "Homeschool Parent's Guide to Teaching Science" is filled with great science experiments and activities. Get your copy by clicking the link below.
A great free resource for really cool science experiments and activities is the Homeschool Science Experiment Guide.
Another good homeschool resource for science ideas, experiments and activities, is the homeschool science blog (just click on the Blog link). Definitely worth bookmarking.
Have Fun!
About the Author Aurora Lipper has been teaching science to kids for over 10 years. She is also a mechanical engineer, university instructor, pilot, astronomer, a real live rocket scientist (You should see the lab in her basement!) and a mom. She has inspired thousands of kids with the fun and magic of science.
Confused...With loads of details...already asked wanted more opinions.?
Ok, so I'm 20 y.o will be 21 in a couple of months, I haven't had regular periods (I'll get one every 4-6 months) and I'm not obese, but I could lose about 40 pounds and I asked my mom, she said when she was heavier she never really got her periods and when she lost weight they started normally,anyway.I have already been pregnant once while with these crazy periods but had a m/c in Dec of 05.My bf and I have been TTC lately.I chart my saliva with a microscope to look for the "ferning" pattern and about 3-4 days ago it did show that I was "ferning" and it looked exactly like the pictures of fertile on the internet...I bought an OPK yesterday and took 1 test yesterday and 1 this morning and they both came out negative (where the control line was darker than the results line) I'm wondering could I have ovulated 3-4 days ago and was just too late with the OPK for it to predict it? or is the "ferning" not right? & can you ovulate w/o getting a period every month (because I got pg once)?
When using the saliva scope you typically see ferning for a couple of days at least. But yes, if you saw ferning 4 days before doing an OPK then chances are you would have already ovulated and therefore the OPK would be neg.
And your question about O'ing without a period, no, this isn't possible.
When the follicle releases the egg then the follicle turns to a corpus luteum and immediately starts producing high amounts of progesterone. This progesterone is what makes your body temp go up, causes the lining of the uterus to thicken, and then, in about 2 weeks, the corpus luteum dies (if not pg) and you get your period. The only way a woman would ovulate and not get a period about 2 weeks later, would be if she were to have conceived that cycle.
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Scale Laboratory
Jun/100
Scale Laboratory
This is essentially starvation. Your body needs a certain number of calories just to cover your basal metabolic rate (BMR) in order to function. This does NOT include daily activities, or intentional exercise. This number (usually you can hit the ballpark if you just take your weight and multiply it by 1000, or just add a zero to your weight in pounds, ex: if you weigh 120, then you should consume 1200 calories just to cover your BMR) is an indication of how much you should consume, just to cover living, breathing, or sitting on the couch. This is for basic human function. Eating has a thermic affect on the body.
If you choose to eat less, you will lose weight, but there are some other information you need arm yourself with in order to make sure it's the kind of weight you seek to lose. Here's what I mean.
If the scale says you weigh less, that does NOT necessarily mean you are doing the right thing.
Here's why.....
THE TRUTH: Eat less weigh less does 3 things:
1. You lose water.
2. You lose very little fat.
3. You lose lots of muscle.
Let me explain further.....
When starving several things happen:
- Your body goes for the metabolically active tissue - muscle.
- It goes into survival mode, and the way it survives is by eating your muscle--it stores fat because that's how it can survive the longest. Your remarkable body doesn't know that fat loss is your goal or what you're trying to do so it seeks for the way it will preserve itself the longest. It wants to help you survive your self-imposed starvation.
- In the meantime, your body continually catabolizes itself (muscle).
- If muscle is eliminated, then it can survive on fewer calories, preserving itself.
- Low-cal diets have no real lifetime affect-it makes you worse off in the long run. It catabolizes your muscle until your brain decides that you will provide it with the necessary nutrition it needs in order to stop feeding on your precious muscle.
- Your body goes into an unhealthy state, driving you to the quickest energy nutrients it needs to survive: FAT & SUGAR! This is a vicious cycle. Your job is to take yourself out of this cycle by first stop starving yourself, and second, ELIMINATE THE SUGAR!
Muscle is good!
o The scale cannot differentiate between fat, muscle, bone, water, the last meal you just ate, clothing, or your internal organs - it simply sums it all up and gives you the grand total. It is how much gravity is pulling on your body at that point and time. The method that gives you the most information is a body caliper test, or body fat test which determines your body fat percentage. This gives you the percentage of fat you have on your body so you can figure out your lean muscle mass. A healthy range for a woman would be between 18 - 23%, Men are between 12 - 15%. You want this number to be low, but your lean body mass (LBM) to be high. You want your body composed of as much LBM as possible.
o Muscle does not weigh more than fat. A pound of muscle weighs the same as a pound of fat. The difference is that muscle takes up far less space on the body than fat and is far more appealing than a pound of fat on your body.
o If you lose weight and the weight loss is primarily muscle, the "loss" will be short lived and unhealthy, provided that your goal is to maintain muscle which is the only site where fat can be burned from your body.
o If seeing the # rise on the scale is stressful to you, get rid of the scale. The muscle that you are working to feed and build will begin to do it's job by burning off the fat on your body. Build the muscle first! Then watch as the fat seems to effortlessly melt from your body.
Velma Garnes M.S, NASM-PES, CPT is a fitness professional and writer who resides in Gahanna, Ohio. She teaches a variety of classes including SPINNING, Ashtanga Yoga, and BootCamp classes and trains clients. Find out more by visiting her website at http://www.focused-fitness.org to access more of her writing and free information.
In addition to the aforementioned certifications, Velma has earned altogether three degrees that have contributed greatly to her success as an excellent fitness trainer: a Bachelor's Degree in Health Administration from Ohio Dominican University and an Associate's Degree in Sport and Exercise Studies from Columbus State Community College. This combined with clinical human performance laboratory work and work experiences she gained while in school, have all given Velma the opportunity to pursue one of her interests - sharing her love and value of fitness with others.
Velma has been inducted into the Sports and Exercise Studies Hall of Fame from Columbus State Community College for her contributions to the program. She has served there as an adjunct faculty member for over 6 years where she taught a myriad of classes including: Total Body Fitness, Components of Aerobic Fitness Training and Intro to Yoga, of which she wrote the coursework for.
Help finding the center of gravity given mass, length and force?
A biology laboratory at your university is studying the location of a person's center of gravity as a function of her or his body weight. They pay well and you decide to volunteer. The location of your center of gravity when standing erect is to be determined by having you lie on a uniform board (mass of 5.00 kg, length 2.00 m) supported by two scales, as shown in the figure. If your height is 190 cm and the left scale reads 430 N while the right scale reads 355 N, where is your center of gravity relative to your feet? Assume the scales are both exactly the same distance from the two ends of the board, are separated by 176 cm, and are set to each read zero before you get on the platform.
Picture to go with problem: http://i284.photobucket.com/albums/ll28/bathtub2008/12-54alt.gif
____cm
I got the answer as 103 cm but I am not very sure about it.
If it is the correct answer, I can provide you with the explanation.
Mail me at your_guide123@yahoo.com
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Vwr Scientific
Jun/100
Vwr Scientific
Want a piece of a multi-billion dollar sales pie? The one served up by the tens of thousands of buyers who make the purchases for Fortune 500 companies and huge government organizations? Then it's time to connect to the world of e-procurement.
It used to be complicated, difficult, and horribly expensive to play the e-procurement connection game. But as Bob Dylan said, "The times, they are a changin'".
What is e-procurement, why does this challenge exist in the first place?
E-Procurement systems are vastly complex, enormously expensive software applications that allow large organizations to ensure that buyers make all their purchases in the same way.
This has several benefits,including:
- Saves buyers time and effort
- Allows management to control purchasing budgets and eliminate maverick spending
- Allows purchasing to interface with other systems within the company including inventory control, shipping and receiving, and accounting
While these systems are a great boon to the company that invests in them, they create a problem for vendors like you and the buyers who need your products and services. That's because e-Procurement systems are internal to the company.
That means that buyers now have a daunting task; get supplier information - like products, prices, and ordering methods -- into their e-procurement system.
That also means the suppliers who want to do business with a company that uses an e-procurement system have to find a way to 'plug in' to their system.
That's exactly why the businesses that have already figured out how to do that are the ones who get the lion's share of these enormous sales. Just take a look at these supply categories and the companies who dominate them:
Office Products: Staples, Office Max, Office Depot
Industrial supplies: Grainger, MSC, HD Supply, Snap-On, Fastenal, IDG
Laboratory equipment and supplies: Fisher Scientific, VWR, Perkin Elmer
Computer equipment and supplies: CDW-G, Apple, Dell, TechDepot, Sharp, PCMall, Lenovo, SHI
Furniture: Steelcase
Follow their lead: There's a reason these companies have dominated for decades. But the good news is that you can level the playing field.
What do all these success stories have in common?
You guessed it; they all connect to the e-procurement of big customers and government organizations.
You see, until recently only businesses with vast technical resources and deep pockets (i.e., giant supply companies) could connect to these complex e-procurement systems.
There a number of companies that are offering these type of connections for far less than they used to be. When you're choosing what company to use make sure you're able to do 3 important things:
Ease of use - you can set up your connection with a simple online wizard. You don't want to have to involve IT guys if you don't need to.
Flexibility - Your setup needs to be adaptable to all the needs of any e-procurement system without having to create multiple separate sites that are not connected to one core central set of data.
An important note: Every e-procurement system out there (and there are dozens) uses a different protocol or language,so if you want to connect to more than one system, you need that flexibility.
Affordability - Because of the fancy terminology that can be used, don't let price stop you from expanding your busines. There are solutions that exist that are very affordable and are a fraction of the cost that other technology companies charge.
But no matter who you use, if you want to compete in today's market, you need to get connected. The sooner you do, the quicker your sales will grow.
You can get more critical information about e-procurement system connections, including some advantages that very few know about, by downloading a free report, "The Power and Profit of PunchOut: How to Cash-In on the New Standard of E-Commerce" at the following link:Â http://www.punchoutconnection.com
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